TBD Turku Research

Research

Tick Biology, Ecology and Evolution

Hard ticks in the genus Ixodes, for instance Ixodes ricinus (the sheep or castor bean tick) and Ixodes persulcatus (the taiga tick), have recently increased in abundance in Finland and elsewhere in northern Europe. This has happened in many locations and under different environments, also in urban and suburban areas nearby big cities. Not all of the reasons for these increases have been examined, but a substantial role for the changing climate has commonly been stressed. Shorter and warmer winters, prolonged growing seasons and other climatic changes are suggested to have direct effects on tick and pathogen performance and indirect effects on tick dynamics via density and range shifts in mammalian and avian host animal populations.

Although several tick species are present, at least occasionally, in Finland, only I. ricinus and I. persulcatus are considered as health threats to humans and domestic animals. These two species look superficially similar, and they may occur at same areas and environments. Both species may carry same bacterial, viral and protozoan pathogens, and they also share hosts in nature: e.g. small rodents, hares, deer, raccoon dogs, passerine birds and humans and their companion animals.

More information:

Ticks and tick-borne pathogens in Finland

Tick Hosts and Pathogen Reservoirs

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Tick Vector Competences

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Experimental TBD Desingns In Vitro

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TBD In Vitro and In Vivo Immunology

Current diagnostic methods for tick-borne CNS infections, have limitations, highlighting the need to develop new diagnostic approaches. This need can be met through the better understanding of immunopathogenesis induced by the causative microbes and the host immune responses.

Various laboratory methods can be used to study the immunopathogenesis and induced host immune responses in tick-borne CNS infections. Single-cell RNA sequencing represents a sophisticated molecular technique facilitating the thorough examination of cellular transcriptomes at a single-cell level. Diverging from conventional flow cytometry approaches, scRNA-seq offers a surface-marker-free analysis for cell identification. Therefore, it serves as a fundamental tool for comprehensive analysis of cellular heterogeneity within populations and the identification of rare cell subsets within a population and it enables gene expression analyses in set-ups with low cellular densities, such as CSF samples.

Contacts:
Jukka Hytönen
Jessica Tikkala
Varpu Rinne
Jukka Alinikula

New Biomarkers and Metabolomics in TBD Diagnostics

Metabolomic analysis of small molecules via UHPLC-MS/MS technique offers a powerful tool for detecting biomarkers in tick-borne diseases like Lyme borreliosis. By analyzing biological sample matrices (e.g., serum, cerebrospinal fluid) from patients with acute infection, this technique reveals unique metabolic profiles associated with the disease. Beyond tick-borne diseases, UHPLC-MS/MS can be applied to other infectious diseases, providing a versatile platform for detecting pathogen-specific biomarkers and pathways. This approach holds promise for differentiating between various infections and tracking disease improvement, opening new avenues for precision diagnostics in infectious disease management, complementing current serology assays.

Contacts:
Jukka Hytönen
Maarit Karonen
Ilari Kuukkanen

Aurum building, Department of Chemistry, University of Turku
Picture: Focus-Video
Copyright: University of Turku

New Antibiotics for Lyme Borreliosis

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Emerging TBD Epidemiology in Finland

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